jeffamine ed 2001 ph 7 0 (Hampton Research Corp)
86
Structured Review
Hampton Research Corp
jeffamine ed 2001 ph 7 0
Jeffamine Ed 2001 Ph 7 0, supplied by Hampton Research Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jeffamine+ed-2001/600+jeffamine+m/pmc12348683-1102-0-5
Average 86 stars, based on 1 article reviews
Jeffamine Ed 2001 Ph 7 0, supplied by Hampton Research Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jeffamine+ed-2001/600+jeffamine+m/pmc12348683-1102-0-5
Average 86 stars, based on 1 article reviews
jeffamine ed 2001 ph 7 0 - by Bioz Stars,
2026-09
86/100 stars
Images
Related Articles
Incubation:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Diffusion-based Assay:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Recombinant:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Filtration:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Planar Chromatography:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Membrane:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Plasmid Preparation:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM Polymerase Chain Reaction:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM In Vitro:Article Title: Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Article Snippet: Diffraction-quality crystals of the DSM25/NKG2D SCD complex were obtained from drops containing 10 mg/mL protein in PNE by hanging-drop vapor diffusion over wells containing 100 mM HEPES (pH 7.2) and 35% Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the former mixing solution, the Zn 2+ -bound EFhd1 core domain (EFhd1 Zn ) crystals were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 2.5 m M ZnSO 4 and 25%( Article Title: Structural and Biochemical Characterization of EFhd1/Swiprosin-2, an Actin-Binding Protein in Mitochondria Article Snippet: Crystals were obtained using a reservoir solution containing 0.1 M HEPES-NaOH (pH 7.5), 5 mM ZnSO 4 , and 25% ( Article Title: Structural Basis for CD96 Immune Receptor Recognition of Nectin-like Protein-5, CD155. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins CD96-D1 This paper N/A Necl-5 (Deuss et al., 2017) N/A Nectin-2 (Deuss et al., 2017) N/A DMEM High Glucose SOBS Media and Prep Services (Monash University) N/A Penicillin-Streptomycin-Glutamine (100X) Gibco Cat# 10378016 Glutamax (100X) Gibco Cat# 35050061 Non-essential Amino Acids (100X) Gibco Cat#11140050 FCS Sigma-Aldrich Cat# F9423 Insect-XPRESS medium Lonza Cat# 12-730Q Polyethyleneimine Sigma-Aldrich Cat# 408727 Cellfectin II Invitrogen Cat# 10362100 Endo H NEB Cat# P0702S D-biotin Sigma-Aldrich Cat# 47868 P20 GE Healthcare Cat# Article Title: Structural and biochemical insights into Zn 2+ -bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Ca 2+ -bound EFhd1 core domain (EFhd1 Ca ) were obtained using reservoir solution containing 0.1 M Tris–HCl (pH 8.5), 0.4 m M ZnSO 4 and 25% Article Title: Biochemical and crystallographic studies of l,d -transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate Article Snippet: Ldt Mt2 wild type or Article Title: Structural and biochemical insights into Zn<sup>2+</sup>-bound EF-hand proteins, EFhd1 and EFhd2 Article Snippet: In the latter mixing solution, crystals of Article Title: Structure and Function of Non-Native Metal Clusters in Human Arginase I Article Snippet: Prepared solutions of 50% (w/v) Jeffamine ED-2001, 100 mM HEPES (pH 7.0) and 30% (v/v) Jeffamine ED-2001, 100 mM |